goscripttm reverse transcription (rt) system (Promega)
90
Structured Review
Promega
goscripttm reverse transcription (rt) system
Goscripttm Reverse Transcription (Rt) System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system/10__1016_slash_j__vas__2025__100471-63-12-17
Average 90 stars, based on 1 article reviews
Goscripttm Reverse Transcription (Rt) System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system/10__1016_slash_j__vas__2025__100471-63-12-17
Average 90 stars, based on 1 article reviews
goscripttm reverse transcription (rt) system - by Bioz Stars,
2026-11
90/100 stars
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Concentration Assay:Article Title: Inhibition of NR2A reduces calcitonin gene-related peptide gene expression induced by cortical spreading depression in rat amygdala. Article Snippet: Despite robust evidence on the role of calcitonin gene-related peptide (CGRP) in migraine via both central and peripheral actions, relatively less is known about how CGRP in the limbic system is involved in migraine progression.. This study investigated whether CGRP production machinery exists in the two key limbic regions including hippocampus and amygdala using cortical spreading depression (CSD) as a model of migraine and whether such alteration by CSD is sensitive to N-methyl-D-aspartate (NMDA) receptor regulation in rats.. A single or repetitive CSD was induced by topical application of KCl and monitored using electrophysiological methods. Reverse Transcription:Article Title: Inhibition of NR2A reduces calcitonin gene-related peptide gene expression induced by cortical spreading depression in rat amygdala. Article Snippet: Despite robust evidence on the role of calcitonin gene-related peptide (CGRP) in migraine via both central and peripheral actions, relatively less is known about how CGRP in the limbic system is involved in migraine progression.. This study investigated whether CGRP production machinery exists in the two key limbic regions including hippocampus and amygdala using cortical spreading depression (CSD) as a model of migraine and whether such alteration by CSD is sensitive to N-methyl-D-aspartate (NMDA) receptor regulation in rats.. A single or repetitive CSD was induced by topical application of KCl and monitored using electrophysiological methods. Article Title: Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection Article Snippet: .. After the amount of total RNA was measured, reverse-transcription of total RNA was performed using the Article Title: Effects of exercise and dietary intervention on muscle, adipose tissue, and blood IRISIN levels in obese male mice and their relationship with the beigeization of white adipose tissue Article Snippet: RNA extraction and RT-PCR analysis of adipose tissue (visceral adipose tissue) and skeletal muscle tissue An RNA extraction reagent, TRIzol (Vazyme Biotech Co., Ltd, Wuhan, China), was used to extract total RNA from the mouse visceral adipose tissue (the amount of s.c. adipose tissue is very small, and the protein content of adipose tissue is low, which cannot meet the needs of the experiment; therefore, visceral adipose tissue was chosen) and skeletal muscle tissues, according to the manufacturer’s instructions. .. The total RNA was then reverse-transcribed into cDNA using a Isolation:Article Title: Aerial Yam Bulbils Protect Against APAP-Induced Hepatotoxicity by Inhibiting Oxidative Stress and Mitochondrial Dysfunction Through Nrf2 Activation Article Snippet: Images were captured using a live-cell imaging system (Oxford Instruments, Oxfordshire, UK). .. Total RNA was isolated using the TRIzol reagent (Sigma). cDNA was synthesized using the Article Title: Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression Article Snippet: .. Total RNA was isolated from cells using Trizol reagent (Invitrogen), and then cDNA was synthesized using Synthesized:Article Title: Aerial Yam Bulbils Protect Against APAP-Induced Hepatotoxicity by Inhibiting Oxidative Stress and Mitochondrial Dysfunction Through Nrf2 Activation Article Snippet: Images were captured using a live-cell imaging system (Oxford Instruments, Oxfordshire, UK). .. Total RNA was isolated using the TRIzol reagent (Sigma). cDNA was synthesized using the Article Title: Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression Article Snippet: .. Total RNA was isolated from cells using Trizol reagent (Invitrogen), and then cDNA was synthesized using other:Article Title: Hypoxia-inducible factor 1α inhibits heat stress-induced pig intestinal epithelial cell apoptosis through eif2α/ATF4/CHOP signaling. Article Snippet: Unstoppable global warming and increased frequency of extreme heat leads to human and animals easier to suffer from heat stress (HS), with gastrointestinal abnormalities as one of the initial clinical symptoms.. HS induces intestinal mucosal damage owing to intestinal hypoxia and hyperthermia.. Hypoxia-inducible factor 1α (HIF-1α) activates numerous genes to mediate cell hypoxic responses; however, its role in HS-treated intestinal mucosa is unknown. |